Cycle sequencing.
نویسندگان
چکیده
منابع مشابه
Multipurpose robot for automated cycle sequencing.
Paramagnetic beads have the superior advantages of easy separation and resuspension by controlling the magnetic filed. Previously, we have developed Magtration technology to automate paramagnetic bead handling and have built several automated instruments that handle 1-12 samples simultaneously. To achieve more high-throughput sample processing, two types of a 96-arrayed Integrated Magtration Un...
متن کاملRapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers.
Numerous methods have been developed for direct sequencing of single or double-stranded DNA amplified by PCR, and most require template purification steps prior to sequencing to remove excess unincorporated primers and dNTPs. Purification methods can be cumbersome, laborious and time consuming, or yield low DNA recoveries. Strategies for direct sequencing of doublestranded DNA products without ...
متن کاملDirect sequencing of RAPD fragments using 3'-extended oligonucleotide primers and dye terminator cycle-sequencing.
Random amplified polymorphic DNA (RAPD) markers are used widely to develop high resolution genetic maps and for genome fingerprinting. Typically, single oligomers of approximately 10 nucleotides are used to PCR amplify characteristic RAPD marker fragments. We describe an efficient method for the direct end-sequencing of gel-purified RAPD fragments using one primer from a set of four 3'-terminal...
متن کاملCycle Sequencing: Dideoxy-mediated Sequencing Reactions Using PCR and End-labeled Primers.
MATERIALS 5x Cycle-sequencing buffer AmpliTaq CS or other exonuclease-deficient versions of Taq DNA polymerase (5 units/μl) EDTA (0.05 M, pH 8.0) Formamide loading buffer Oligonucleotide Primers, radiolabeled at the 5' terminus with 33P or 32P To prepare end-labeled primers, please see Phosphorylating the 5' Termini of Oligonucleotides. Template DNAs Plasmids, cosmids, bacteriophage , and bacte...
متن کاملImproved DNA sequencing quality and efficiency using an optimized fast cycle sequencing protocol.
Vol. 43 ı No. 1 ı 2007 Technological advances in DNA sequencing over the past 20 years have given rise to the science of genomics and have enabled critical advances in other fields, including epidemiology, forensics, evolutionary biology, and medical diagnostics. Further advances in this technology will ensure the proliferation of new applications, such as comparative sequencing, and stimulate ...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1994
ISSN: 1088-9051
DOI: 10.1101/gr.3.5.s107